| Gene | Wfikkn2 |
| Chromosome | 11 |
| Deletion Start | 94,242,546 |
| Deletion End | 94,237,523 |
| Deletion Size | 5,024 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,241,738 | 94,241,758 | CTCGTGGATGCTGCTGTAGTC |
| TUP | 94,241,712 | 94,241,737 | AAGAGCCACCTCCAGACCTTCTTCCC |
| TUR | 94,241,662 | 94,241,680 | TGGGCAGGAGTGAACAAGG |
| TDF | 94,238,273 | 94,238,290 | GACGCCCAGGCTAACAAC |
| TDP | 94,238,244 | 94,238,270 | CCTCACTTTCACCTTTGGTCACTGCCA |
| TDR | 94,238,210 | 94,238,230 | GGCCTCGTAGGTCTCAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGTTCCTCTCCCTACGC |
| SD | TGGAACCCAAAGAACCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12167A-B10 | VGB6 | C57BL/6NTac | |
| 12167A-C1 | VGB6 | C57BL/6NTac | |
