Gene | Unc5b |
Chromosome | 10 |
Deletion Start | 60,783,268 |
Deletion End | 60,765,110 |
Deletion Size | 18,159 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 60,783,176 | 60,783,199 | CAGGACGCCTACATCGTAAAGAAC |
TUP | 60,783,141 | 60,783,164 | ACTGCACTGCAGAGCCTTCCCTGC |
TUR | 60,783,105 | 60,783,125 | ACCCACTCGCCATTACACTTG |
TDF | 60,765,805 | 60,765,825 | CCAGGAGCTTACAGGGACTTC |
TDP | 60,765,782 | 60,765,804 | CCCTGTCTGTGCTTCCCAGCTCC |
TDR | 60,765,759 | 60,765,779 | GTTCCTTGAGCACGCCATTTC |
PCR Primers
Primer | Sequence |
SU | TCCTGGATGCCTCTGTGTC |
SD | CAGTTTGCCTGAACTGAACTC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 95 bp | + | + | - |
TDF | TDR | 67 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
12168A-G8 | VGB6 | C57BL/6NTac | |
12168A-H12 | VGB6 | C57BL/6NTac | |