| Gene | Bmp8b |
| Chromosome | 4 |
| Deletion Start | 86,149,644 |
| Deletion End | 86,157,048 |
| Deletion Size | 7,405 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,105,386 | 123,105,404 | CCTGGCTCTGTGCGTGTTG |
| TUP | 123,105,407 | 123,105,427 | CGGCGGTCACCTCTCGCATCC |
| TUR | 123,105,474 | 123,105,492 | GAATCTCGCGCTGCATGTC |
| TDF | 123,105,561 | 123,105,581 | TCTGCGCCCCTCTTTATGTTG |
| TDP | 123,105,584 | 123,105,607 | CCTGTACCGTGCCATGACGGATGA |
| TDR | 123,105,633 | 123,105,652 | AGGTCAGCACGGTCCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CATTGGTTGAGTTCACTAAGG |
| SD | AGCACTCAGACCTTCCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12170A-H10 | VGB6 | C57BL/6NTac | |
| 12170A-G8 | VGB6 | C57BL/6NTac | |
| 12170A-C3 | VGB6 | C57BL/6NTac | |
| 12170A-C2 | VGB6 | C57BL/6NTac | |
| 12170A-C5 | VGB6 | C57BL/6NTac | |
