| Gene | Bmp8a |
| Chromosome | 4 |
| Deletion Start | 123,313,175 |
| Deletion End | 123,312,861 |
| Deletion Size | 315 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,342,675 | 123,342,693 | GGGCCACTCTGGCTATTGG |
| TUP | 123,342,653 | 123,342,672 | CTTGCTCTGTGCGCGCTGGG |
| TUR | 123,342,557 | 123,342,575 | AGCACCGCCAGGATTTCAC |
| TDF | 123,342,475 | 123,342,494 | CGCCCCTCTTCATGTTGGAC |
| TDP | 123,342,449 | 123,342,470 | ACCACGCCATGACCGATGACGA |
| TDR | 123,342,418 | 123,342,437 | GCCTAAGTGAGCCTGTGGTG |
PCR Primers
| Primer | Sequence |
| SU | TGCGGACACTCGCAAGTCA |
| SD | CCTGGACATCTCCAGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12171A-F5 | VGB6 | C57BL/6NTac | |
| 12171A-E5 | VGB6 | C57BL/6NTac | |
| 12171A-H12 | VGB6 | C57BL/6NTac | |
