| Gene | Mmp9 |
| Chromosome | 2 |
| Deletion Start | 164,948,260 |
| Deletion End | 164,954,913 |
| Deletion Size | 6,654 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,949,003 | 164,949,022 | GACGTGGGTCGATTCCAAAC |
| TUP | 164,949,025 | 164,949,049 | TCAAAGGCCTCAAGTGGGACCATCA |
| TUR | 164,949,067 | 164,949,087 | CTGCTTCTAGCCCGACATCTC |
| TDF | 164,954,765 | 164,954,786 | TCCCGTAGACAAAGCCTATTTC |
| TDP | 164,954,789 | 164,954,814 | CCATGGCAAATTCTTCTGGCGTGTGA |
| TDR | 164,954,831 | 164,954,852 | CACCTCATGGTCCACCTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTCAGAAGCCCAAGGAAGAG |
| SD | TCAATTCTCAGACGCCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12174A-A3 | VGB6 | C57BL/6NTac | |
| 12174A-E3 | VGB6 | C57BL/6NTac | |
| 12174A-F12 | VGB6 | C57BL/6NTac | |
| 12174A-E9 | VGB6 | C57BL/6NTac | |
