| Gene | Gip |
| Chromosome | 11 |
| Deletion Start | 96,025,379 |
| Deletion End | 96,030,744 |
| Deletion Size | 5,366 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,026,047 | 96,026,066 | TGGACCAAAGCCAAGACAAC |
| TUP | 96,026,068 | 96,026,092 | ACAGGACTGGCTAATGCTGAGCCTG |
| TUR | 96,026,123 | 96,026,145 | TTTCAAACCTCCTGCCTCTACTG |
| TDF | 96,029,797 | 96,029,818 | TGAGGGTCGAAATACTGTGGAG |
| TDP | 96,029,819 | 96,029,846 | TGACCCATGTACCCTATGGTGAAGAGGA |
| TDR | 96,029,860 | 96,029,879 | GGTGAGTTTGGGCTTCTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTAATGAGCCAGGAAAGG |
| SD | TGTTAGCAATCAGGGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12175A-B9 | VGB6 | C57BL/6NTac | |
| 12175A-G5 | VGB6 | C57BL/6NTac | |
| 12175A-E12 | VGB6 | C57BL/6NTac | |
