| Gene | Lin7b |
| Chromosome | 7 |
| Deletion Start | 45,370,555 |
| Deletion End | 45,367,899 |
| Deletion Size | 2,657 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,369,980 | 45,369,999 | CCCGACTTCCCAGGTGTATG |
| TUP | 45,369,952 | 45,369,979 | AACAGCTCTATGACACGCTGGACATCAC |
| TUR | 45,369,896 | 45,369,914 | TACCGGAGCAGGAGCTTAC |
| TDF | 45,368,304 | 45,368,323 | CAGCAGCACCACAGCTACAC |
| TDP | 45,368,278 | 45,368,302 | TGAGCCTCCAGTCACCATCCTCTCC |
| TDR | 45,368,236 | 45,368,260 | CACTGGCCTGATTTGGGTATCTAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGTCTTCCAAGTCACAGCG |
| SD | CTGCTGGTTTCAAGACTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12182A-A1 | VGB6 | C57BL/6NTac | |
| 12182A-H3 | VGB6 | C57BL/6NTac | |
| 12182A-D10 | VGB6 | C57BL/6NTac | |
| 12182A-E2 | VGB6 | C57BL/6NTac | |
| 12182A-B8 | VGB6 | C57BL/6NTac | |
| 12182A-B12 | VGB6 | C57BL/6NTac | |
