| Gene | Cetn2 |
| Chromosome | X |
| Deletion Start | 72,918,313 |
| Deletion End | 72,914,132 |
| Deletion Size | 4,182 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,917,926 | 72,917,949 | AAGAGGTTTACTGTGTTCTGATGG |
| TUP | 72,917,903 | 72,917,925 | TCGCAAACGCTTTCAGGAGAGGC |
| TUR | 72,917,882 | 72,917,899 | GCACCTGCGTGCTTTCTC |
| TDF | 72,914,758 | 72,914,782 | GGAAAGCTAGTATCCCTCATACCTG |
| TDP | 72,914,714 | 72,914,742 | ACCTGTGGACCCTGTTTCGATAATACACA |
| TDR | 72,914,664 | 72,914,683 | TGGAAAGTCACCCTGACCTG |
PCR Primers
| Primer | Sequence |
| SU | CTTCCTCCAGGCTCTGTCAC |
| SD | AATGCCACAGCAAGCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12185A-A7 | VGB6 | C57BL/6NTac | |
| 12185A-F8 | VGB6 | C57BL/6NTac | |
| 12185A-H8 | VGB6 | C57BL/6NTac | |
