| Gene | Zfp828 |
| Chromosome | 8 |
| Deletion Start | 13,877,848 |
| Deletion End | 13,880,259 |
| Deletion Size | 2,412 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,878,053 | 13,878,072 | CAGCAAGCTGTACGCCAATG |
| TUP | 13,878,076 | 13,878,100 | ACTATCACATCACGGCCAGACACGC |
| TUR | 13,878,118 | 13,878,135 | GCTCTTTCGGCTGCTCAC |
| TDF | 13,879,673 | 13,879,691 | GAAGGACAGCCAGGAGAAC |
| TDP | 13,879,692 | 13,879,714 | TCGGATGCCGAGCTCAGTAGCAG |
| TDR | 13,879,724 | 13,879,746 | CCAACGTGTCTAAATCTGCTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGGGAAGGTACGGCTATGTC |
| SD | ACACACACACATGGTGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12186A-A2 | VGB6 | C57BL/6NTac | |
| 12186A-H5 | VGB6 | C57BL/6NTac | |
| 12186A-C5 | VGB6 | C57BL/6NTac | |
| 12186A-F6 | VGB6 | C57BL/6NTac | |
| 12186A-G9 | VGB6 | C57BL/6NTac | |
| 12186A-E1 | VGB6 | C57BL/6NTac | |
