| Gene | Cldn1 |
| Chromosome | 16 |
| Deletion Start | 26,371,623 |
| Deletion End | 26,358,954 |
| Deletion Size | 12,670 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,371,302 | 26,371,321 | GGAGCCCTTGTCCTCTGTAG |
| TUP | 26,371,276 | 26,371,300 | AGCTTCATTCAGCCCAGAGGCTACC |
| TUR | 26,371,212 | 26,371,235 | GGCTTGGGTTCTTTCTGCTTTATC |
| TDF | 26,359,735 | 26,359,754 | AGGCAAAGCTGCCAGTTCAG |
| TDP | 26,359,707 | 26,359,732 | CTGCAGCAGCTGAACTTGAGTCTGGA |
| TDR | 26,359,634 | 26,359,658 | GATGAGGAGTCTGTGGTTGACTATG |
PCR Primers
| Primer | Sequence |
| SU | ACGAAGGGAAGAGGTTAGGG |
| SD | TGTGCTGGTACAGTATGAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12187A-F8 | VGB6 | C57BL/6NTac | |
| 12187B-C9 | VGB6 | C57BL/6NTac | |
| 12187B-H2 | VGB6 | C57BL/6NTac | |
| 12187B-G6 | VGB6 | C57BL/6NTac | |
