| Gene | Zfp597 |
| Chromosome | 16 |
| Deletion Start | 3,872,088 |
| Deletion End | 3,865,611 |
| Deletion Size | 6,478 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,871,923 | 3,871,946 | GGCTTACCGACTTCCTCATTTCAG |
| TUP | 3,871,895 | 3,871,921 | ATCATCAGGTGGAGGAGAGCCCTAGCA |
| TUR | 3,871,841 | 3,871,862 | GCCATTTGGAGACGCATTTCAG |
| TDF | 3,866,290 | 3,866,309 | CCGAACCAACCTGAGGACAC |
| TDP | 3,866,262 | 3,866,288 | TAAGCGAATCCACACTGGTGAGAAGCC |
| TDR | 3,866,231 | 3,866,248 | GAAGCTGGCAGCACACTC |
PCR Primers
| Primer | Sequence |
| SU | CAATCAGGATGCAGGATGG |
| SD | TATATCATGTAATAGGACTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12188A-A4 | VGB6 | C57BL/6NTac | |
| 12188A-B3 | VGB6 | C57BL/6NTac | |
| 12188A-H2 | VGB6 | C57BL/6NTac | |
| 12188A-D3 | VGB6 | C57BL/6NTac | |
| 12188A-G4 | VGB6 | C57BL/6NTac | |
| 12188A-C3 | VGB6 | C57BL/6NTac | |
