| Gene | Plag1 |
| Chromosome | 4 |
| Deletion Start | 3,905,685 |
| Deletion End | 3,903,667 |
| Deletion Size | 2,019 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,904,791 | 3,904,814 | TGTGGCAAGAGCTACAATACCAAG |
| TUP | 3,904,757 | 3,904,782 | TAAGCGACACTTGGCCTTGCATGCTG |
| TUR | 3,904,734 | 3,904,752 | GCAGGTGAGGTCACCACTG |
| TDF | 3,904,504 | 3,904,523 | GGACGAAAGGATCACCTCAC |
| TDP | 3,904,474 | 3,904,503 | TCGACACATGAAGAAGAGTCACAATCAGGA |
| TDR | 3,904,423 | 3,904,445 | CAGGTAAATGGGTCCAGGAAATC |
PCR Primers
| Primer | Sequence |
| SU | CCTTTAGAACAATGCTTTGGG |
| SD | CCAATGCCCATTGCTATTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12189A-D3 | VGB6 | C57BL/6NTac | |
| 12189A-D8 | VGB6 | C57BL/6NTac | |
| 12189B-A3 | VGB6 | C57BL/6NTac | |
| 12189B-A12 | VGB6 | C57BL/6NTac | |
| 12189B-B4 | VGB6 | C57BL/6NTac | |
| 12189B-D2 | VGB6 | C57BL/6NTac | |
| 12189B-E2 | VGB6 | C57BL/6NTac | |
| 12189B-E8 | VGB6 | C57BL/6NTac | |
