| Gene | Mia1 |
| Chromosome | 7 |
| Deletion Start | 27,181,042 |
| Deletion End | 27,179,796 |
| Deletion Size | 1,247 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,180,842 | 27,180,864 | CAGTTGCTGTCATTTCCTTCTCC |
| TUP | 27,180,812 | 27,180,837 | CCAGATCCTATCTCCATGGCTGTGGC |
| TUR | 27,180,773 | 27,180,792 | GTCAAGAAGCGGCAATCAGG |
| TDF | 27,180,010 | 27,180,030 | TGAGGGCTGCTGAGTTTACAG |
| TDP | 27,179,985 | 27,180,008 | CCCTGCCCAGTGCTGAGTGATCTC |
| TDR | 27,179,958 | 27,179,977 | ACTGCACCCTCTGCCATTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGCTCAGTACCTGGGAACC |
| SD | TACGTTCTATGCGCTTCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12190B-G2 | VGB6 | C57BL/6NTac | |
| 12190B-F2 | VGB6 | C57BL/6NTac | |
