| Gene | Rnf181 |
| Chromosome | 6 |
| Deletion Start | 72,362,362 |
| Deletion End | 72,360,564 |
| Deletion Size | 1,799 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,362,159 | 72,362,176 | TCAGGGCGACGCGATTAG |
| TUP | 72,362,134 | 72,362,158 | TCGAGTCGGAGTCGTTAATTTGGGC |
| TUR | 72,362,070 | 72,362,090 | GCTACACCCACCCTTTCTATC |
| TDF | 72,360,979 | 72,360,998 | GGAAGGCAGGAGCAGGAAAG |
| TDP | 72,360,947 | 72,360,976 | CCGGAATACCGCTCTTGATTTCTCTAGACA |
| TDR | 72,360,918 | 72,360,936 | AGCTCATGGCGGCACAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCAGGTAAACTCAGCCAAGG |
| SD | AGAACACTCAGACCCAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12199B-B6 | VGB6 | C57BL/6NTac | |
| 12199B-H7 | VGB6 | C57BL/6NTac | |
| 12199B-C3 | VGB6 | C57BL/6NTac | |
| 12199B-F8 | VGB6 | C57BL/6NTac | |
| 12199B-D10 | VGB6 | C57BL/6NTac | |
