| Gene | Drap1 |
| Chromosome | 19 |
| Deletion Start | 5,424,711 |
| Deletion End | 5,422,858 |
| Deletion Size | 1,854 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,424,238 | 5,424,258 | TTCCTGGAGTCCCTGTTGAAG |
| TUP | 5,424,215 | 5,424,237 | AAGGCTTGCCAGGTGACCCAGTC |
| TUR | 5,424,131 | 5,424,150 | TGTCGGAACTCTGCCTCCTC |
| TDF | 5,423,590 | 5,423,611 | GGGAAGCAAAGGCAAAGACAAG |
| TDP | 5,423,564 | 5,423,585 | TGTCCGGGACAGACTCTGAGCA |
| TDR | 5,423,538 | 5,423,559 | ATCACCCATCAGCCTCACTCAC |
PCR Primers
| Primer | Sequence |
| SU | AACGTTCGTACTTTAGCTTGGG |
| SD | TTCAAGGCCCAGTCTCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12200A-A6 | VGB6 | C57BL/6NTac | |
| 12200A-A10 | VGB6 | C57BL/6NTac | |
| 12200A-F6 | VGB6 | C57BL/6NTac | |
| 12200A-F10 | VGB6 | C57BL/6NTac | |
| 12200A-H2 | VGB6 | C57BL/6NTac | |
| 12200A-F7 | VGB6 | C57BL/6NTac | |
