| Gene | Zbtb48 |
| Chromosome | 4 |
| Deletion Start | 152,027,053 |
| Deletion End | 152,019,759 |
| Deletion Size | 7,295 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,026,343 | 152,026,366 | GGAGGCAAGCATAAGGGTAAAGAC |
| TUP | 152,026,316 | 152,026,342 | CGACACTTCGGGAGCAAACATGGGAAG |
| TUR | 152,026,276 | 152,026,297 | GGACAAGTGTAGCTCGCAGAAG |
| TDF | 152,020,512 | 152,020,534 | AGGCAAGCTGTAGGAGAATAGTG |
| TDP | 152,020,487 | 152,020,508 | CTGGCTTGTTTCCTGCCCGCCT |
| TDR | 152,020,453 | 152,020,474 | GGTGCCCTTGATCTCTGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | CTGTCCGTTGGGTGCTCTAC |
| SD | TAATTCTGGCCAATGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12201B-B3 | VGB6 | C57BL/6NTac | |
| 12201B-B7 | VGB6 | C57BL/6NTac | |
| 12201B-G7 | VGB6 | C57BL/6NTac | |
| 12201B-G9 | VGB6 | C57BL/6NTac | |
| 12201B-A10 | VGB6 | C57BL/6NTac | |
