| Gene | BC089491 |
| Chromosome | 7 |
| Deletion Start | 28,291,074 |
| Deletion End | 28,287,948 |
| Deletion Size | 3,127 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,290,168 | 28,290,186 | GGGAGGCAGATCCACAGAG |
| TUP | 28,290,140 | 28,290,166 | TCTGACCACCACTTGTCTGTCCCTTTG |
| TUR | 28,290,113 | 28,290,133 | AAAGAAGCCAGGGTGGGAAAC |
| TDF | 28,288,494 | 28,288,511 | TGCTCTCCGCTTGCAGTG |
| TDP | 28,288,467 | 28,288,491 | CTCTGAAGCTATGGCCTTCGGGTGA |
| TDR | 28,288,428 | 28,288,451 | CCTACCATTTGCTCCCTTACATTC |
PCR Primers
| Primer | Sequence |
| SU | CAATGGGAAGCTCTAGGCAG |
| SD | TGGGGAGCAGTGATGGGGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12203A-F3 | VGB6 | C57BL/6NTac | |
| 12203A-H7 | VGB6 | C57BL/6NTac | |
| 12203A-H9 | VGB6 | C57BL/6NTac | |
