| Gene | Ube2a |
| Chromosome | X |
| Deletion Start | 36,874,489 |
| Deletion End | 36,883,148 |
| Deletion Size | 8,660 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,875,271 | 36,875,292 | CCACTTAAAGAAGCGGCTTTGC |
| TUP | 36,875,293 | 36,875,316 | CCCTGCCACCTCTGACCACCAAAG |
| TUR | 36,875,320 | 36,875,342 | CGCCACACACTGAATTTCACTTG |
| TDF | 36,883,019 | 36,883,043 | TGGATGAACCTAATCCCAATAGTCC |
| TDP | 36,883,044 | 36,883,064 | AGCAAACAGCCAGGCTGCCCA |
| TDR | 36,883,066 | 36,883,086 | CCGCTTGTTCTCCTGGTACAG |
PCR Primers
| Primer | Sequence |
| SU | CAGGTCAACTTTGGCTCTCC |
| SD | GCTTTGAGGCTGGAGATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12204A-A3 | VGB6 | C57BL/6NTac | |
| 12204A-A5 | VGB6 | C57BL/6NTac | |
| 12204A-A12 | VGB6 | C57BL/6NTac | |
| 12204A-C12 | VGB6 | C57BL/6NTac | |
| 12204A-G7 | VGB6 | C57BL/6NTac | |
| 12204A-H9 | VGB6 | C57BL/6NTac | |
