| Gene | Zfp687 |
| Chromosome | 3 |
| Deletion Start | 95,012,456 |
| Deletion End | 95,007,430 |
| Deletion Size | 5,027 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,011,635 | 95,011,653 | CCAGAGTCCTCCTGCTTCC |
| TUP | 95,011,609 | 95,011,634 | CCTGTCAAGGCACCCAGCTGTAAACC |
| TUR | 95,011,575 | 95,011,595 | CTTGTCCACTGTTCCCTCATC |
| TDF | 95,008,244 | 95,008,263 | GGTCTTTGCATGTGCCTCTG |
| TDP | 95,008,216 | 95,008,242 | TGCCATCTTGCCCACTTTACCTCTGCT |
| TDR | 95,008,187 | 95,008,205 | AAGGTGCGCTTTCCCTCTG |
PCR Primers
| Primer | Sequence |
| SU | CCCAGAGCAGTATGTCTGCC |
| SD | ACTAGGACAGAACCCAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12213A-A12 | VGB6 | C57BL/6NTac | |
| 12213A-B10 | VGB6 | C57BL/6NTac | |
