| Gene | Birc3 |
| Chromosome | 9 |
| Deletion Start | 7,861,306 |
| Deletion End | 7,849,235 |
| Deletion Size | 12,072 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,860,902 | 7,860,924 | CTGGCTATTTCAGTGGCTCTTAC |
| TUP | 7,860,877 | 7,860,900 | CGAGCTTTCCCTCAGACCCTGTGA |
| TUR | 7,860,840 | 7,860,859 | GTGCTCAAAGCAGGACAATC |
| TDF | 7,849,376 | 7,849,395 | GGACCGAGAGGTATCCATCG |
| TDP | 7,849,350 | 7,849,375 | TGTTCATTCCCTGTGGCCATCTGGTC |
| TDR | 7,849,331 | 7,849,349 | GAGCGCAGTCTTTGCACAC |
PCR Primers
| Primer | Sequence |
| SU | CCAAGCTTCAGAATGTGCTTC |
| SD | CACATGGACATCTGTGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12218A-A1 | VGB6 | C57BL/6NTac | |
| 12218A-E3 | VGB6 | C57BL/6NTac | |
