| Gene | Pdzd11 |
| Chromosome | X |
| Deletion Start | 100,625,465 |
| Deletion End | 100,623,450 |
| Deletion Size | 2,016 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,625,163 | 100,625,181 | GTGACCCACTGGTGACTTC |
| TUP | 100,625,138 | 100,625,161 | TGCAGAATCACCATGCCAGAACCC |
| TUR | 100,625,089 | 100,625,112 | GACAGGATGGAAATTAAGGCTCTG |
| TDF | 100,624,253 | 100,624,275 | GGCAGACAATGGTGACATAGGAC |
| TDP | 100,624,223 | 100,624,246 | CCCAGCACATGGGAGGCAGAGATA |
| TDR | 100,624,170 | 100,624,191 | CCCTGGAACTCACTCCGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGTGAGAAATGCAAACATGCC |
| SD | TTAGCTGTATAAGTGACCAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12222A-C1 | VGB6 | C57BL/6NTac | |
| 12222A-G11 | VGB6 | C57BL/6NTac | |
