| Gene | Agtr2 |
| Chromosome | X |
| Deletion Start | 21,486,132 |
| Deletion End | 21,487,226 |
| Deletion Size | 1,095 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,486,150 | 21,486,167 | GCTGCCACCAGCAGAAAC |
| TUP | 21,486,171 | 21,486,198 | ACCAGCAGCCGTCCTTTTGATAATCTCA |
| TUR | 21,486,202 | 21,486,222 | CGGACTCATTGGTGCCAGTTG |
| TDF | 21,486,905 | 21,486,926 | TGCTGTTGTGTTGGCATTCATC |
| TDP | 21,486,930 | 21,486,954 | TGCTGGCTTCCCTTCCATGTTCTGA |
| TDR | 21,486,956 | 21,486,977 | CCAGGTCAGAGCATCCAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | ATGACTCCTACCCTGATTAAC |
| SD | AGGGAGAAGTCTGAGATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12224B-C1 | VGB6 | C57BL/6NTac | |
| 12224B-C11 | VGB6 | C57BL/6NTac | |
| 12224B-D10 | VGB6 | C57BL/6NTac | |
| 12224B-H10 | VGB6 | C57BL/6NTac | |
| 12224C-A5 | VGB6 | C57BL/6NTac | |
| 12224C-B9 | VGB6 | C57BL/6NTac | |
