| Gene | Lrrc8d |
| Chromosome | 5 |
| Deletion Start | 105,811,729 |
| Deletion End | 105,814,306 |
| Deletion Size | 2,578 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,811,998 | 105,812,017 | CGGGCAGACGACAACGAATG |
| TUP | 105,812,018 | 105,812,039 | ACGTTGCCTTTGGCACATCCGC |
| TUR | 105,812,052 | 105,812,073 | ATGGGTGGCTTGGAGAGGTATG |
| TDF | 105,814,181 | 105,814,200 | GCCAGTGTCGGATGCTCAAG |
| TDP | 105,814,201 | 105,814,228 | AAGAGCGGGCTTGTTGTAGAAGACCAAC |
| TDR | 105,814,230 | 105,814,250 | TCTAGTGGCAGCGTGTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTCCTGATACTTCTCTG |
| SD | TTTAGCAGGCAAGCAATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12225A-B6 | VGB6 | C57BL/6NTac | |
| 12225A-B9 | VGB6 | C57BL/6NTac | |
| 12225A-D8 | VGB6 | C57BL/6NTac | |
| 12225A-D9 | VGB6 | C57BL/6NTac | |
| 12225A-F4 | VGB6 | C57BL/6NTac | |
| 12225A-G3 | VGB6 | C57BL/6NTac | |
