| Gene | Gpr17 |
| Chromosome | 18 |
| Deletion Start | 31,948,005 |
| Deletion End | 31,946,973 |
| Deletion Size | 1,033 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,947,880 | 31,947,903 | TGCTTCTACCTTCTGGACTTCATC |
| TUP | 31,947,850 | 31,947,869 | TGGGCAATGCTCTGGCCCTG |
| TUR | 31,947,813 | 31,947,831 | CCGGAGTGCCTGACTTGTG |
| TDF | 31,947,445 | 31,947,464 | TGCAACTGTACCGGGAGAAG |
| TDP | 31,947,418 | 31,947,439 | CCATCACGCCCTGGCATCCCTG |
| TDR | 31,947,376 | 31,947,394 | GCAGGTGACCGTGGTGATG |
PCR Primers
| Primer | Sequence |
| SU | GCACTAACTCCACCCTCTCG |
| SD | GGGAGCTGTACAGAAGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12229B-B5 | VGB6 | C57BL/6NTac | |
| 12229B-C3 | VGB6 | C57BL/6NTac | |
| 12229E-A3 | VGB6 | C57BL/6NTac | |
| 12229E-D3 | VGB6 | C57BL/6NTac | |
| 12229E-G8 | VGB6 | C57BL/6NTac | |
| 12229E-F3 | VGB6 | C57BL/6NTac | |
| 12229E-F1 | VGB6 | C57BL/6NTac | |
