| Gene | Nfil3 |
| Chromosome | 13 |
| Deletion Start | 52,968,863 |
| Deletion End | 52,967,464 |
| Deletion Size | 1,400 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,968,225 | 52,968,243 | CCGGAGCCCTGAGAACAAG |
| TUP | 52,968,201 | 52,968,223 | TCCCTGTGATCAAGCAGGAGCCC |
| TUR | 52,968,177 | 52,968,197 | CCTGGCAAAGCTCTCCAACTC |
| TDF | 52,968,093 | 52,968,114 | TTTCTCCACTTACTCCCACTCC |
| TDP | 52,968,066 | 52,968,088 | CCCTCTTGCAGGTCCATGGGTCC |
| TDR | 52,968,016 | 52,968,035 | TTGCCCACTACACCCTCATC |
PCR Primers
| Primer | Sequence |
| SU | AGCACTCCTCTCTCTGCTGG |
| SD | GATCAAGTGGAACAGAGCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12235B-E6 | VGB6 | C57BL/6NTac | |
| 12235C-E10 | VGB6 | C57BL/6NTac | |
| 12235C-E1 | VGB6 | C57BL/6NTac | |
