12237   Hyal3     Status: ES cell colonies screened / QC positives

GeneHyal3
Chromosome9
Deletion Start107,584,772
Deletion End107,586,925
Deletion Size2,154
Genome Build38
MGI IDMGI:1330288
NCBI Gene ID109685
BAC ID356N18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF107,585,213107,585,231GCCTCCTGGGTTTGGACAC
TUP107,585,234107,585,259CAGATGTTCCCTGGCTTGGATCCTCA
TUR107,585,261107,585,280TGGGCTTTGTGGAGCTGTTC
TDF107,586,704107,586,725CCAGGACAAATGGAAGCCTTTC
TDP107,586,730107,586,754TCTGCAGCCAGATGACAGTCTTGGA
TDR107,586,776107,586,797CAGCCCAACCCGAATAACAATG

PCR Primers

PrimerSequence
SUTATGGATGAAGCAAGGAGGC
SDAGCTTAGCGTTTAACAGACCTTAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR68 bp++-
TDFTDR94 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12237B-D9VGB6C57BL/6NTac
12237B-E2VGB6C57BL/6NTac
12237C-B5VGB6C57BL/6NTac
12237C-D11VGB6C57BL/6NTac
12237C-H4VGB6C57BL/6NTac
12237C-H9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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