| Gene | Bach1 |
| Chromosome | 16 |
| Deletion Start | 87,715,394 |
| Deletion End | 87,729,937 |
| Deletion Size | 14,544 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,715,408 | 87,715,428 | TGCGGTATTTGCCTACGAGTC |
| TUP | 87,715,430 | 87,715,454 | TCTGTGCATAGCACCAACGTCTTGC |
| TUR | 87,715,462 | 87,715,482 | TCCTTCTTCCGCTGGTCATTG |
| TDF | 87,729,503 | 87,729,524 | CAGAACCTGACCGGACTTTGTC |
| TDP | 87,729,528 | 87,729,549 | AGGTGTGCAAGGAAGCCGCCCT |
| TDR | 87,729,557 | 87,729,577 | GGCAAGGATCTGGATCTGCTC |
PCR Primers
| Primer | Sequence |
| SU | GACGGGATTCTGACTGAAGG |
| SD | GCTGGACTTTGAAGGAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12241A-A8 | VGB6 | C57BL/6NTac | |
| 12241A-H9 | VGB6 | C57BL/6NTac | |
| 12241A-H6 | VGB6 | C57BL/6NTac | |
| 12241A-H8 | VGB6 | C57BL/6NTac | |
| 12241A-H2 | VGB6 | C57BL/6NTac | |
| 12241A-D11 | VGB6 | C57BL/6NTac | |
