| Gene | Lrrc3 |
| Chromosome | 10 |
| Deletion Start | 77,901,597 |
| Deletion End | 77,900,792 |
| Deletion Size | 806 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,901,394 | 77,901,412 | AGCCGACACGGTGCTTCTG |
| TUP | 77,901,363 | 77,901,390 | CTAGACGCCAACAGAATCTCCCGAGTCC |
| TUR | 77,901,314 | 77,901,333 | GACAGGTCCAGCTCCCTCAG |
| TDF | 77,900,878 | 77,900,898 | CGACACCTGGAGTACCTGAAG |
| TDP | 77,900,852 | 77,900,875 | CCTGCCTAGTGCTCCAGTCTCCAA |
| TDR | 77,900,828 | 77,900,846 | TAGGGCACAGGGCTAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTATGGGTGGGTGACCAGAG |
| SD | ATTGCCGGTACTTTGCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12244A-B8 | VGB6 | C57BL/6NTac | |
| 12244A-C1 | VGB6 | C57BL/6NTac | |
| 12244A-C2 | VGB6 | C57BL/6NTac | |
| 12244A-E4 | VGB6 | C57BL/6NTac | |
| 12244A-D6 | VGB6 | C57BL/6NTac | |
| 12244A-G3 | VGB6 | C57BL/6NTac | |
