| Gene | Atn1 |
| Chromosome | 6 |
| Deletion Start | 124,749,770 |
| Deletion End | 124,743,041 |
| Deletion Size | 6,730 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,749,665 | 124,749,684 | AAGGGCTTGGAGCGGAGAAC |
| TUP | 124,749,636 | 124,749,663 | CAGGCAGCATAGAGCCTAAAGTTAGGGC |
| TUR | 124,749,604 | 124,749,625 | GAGCGGGATGGTACAGGTAATG |
| TDF | 124,743,109 | 124,743,131 | TCATCTCTGCATTGCCTCTTATC |
| TDP | 124,743,079 | 124,743,105 | TCACCTGAAGAAGGAGAGTGACAAGCC |
| TDR | 124,743,056 | 124,743,075 | TGTCTGGATCGCAGCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | TGTGGAGGGTAAAGTCCTGC |
| SD | ATCACACCAATGGGTTAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12251A-A3 | VGB6 | C57BL/6NTac | |
| 12251A-A9 | VGB6 | C57BL/6NTac | |
| 12251A-B4 | VGB6 | C57BL/6NTac | |
| 12251A-F3 | VGB6 | C57BL/6NTac | |
| 12251A-D9 | VGB6 | C57BL/6NTac | |
| 12251A-A11 | VGB6 | C57BL/6NTac | |
