| Gene | Lag3 |
| Chromosome | 6 |
| Deletion Start | 124,911,348 |
| Deletion End | 124,904,364 |
| Deletion Size | 6,985 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,911,300 | 124,911,320 | TTCTGGGACTGCTTTGGGAAG |
| TUP | 124,911,274 | 124,911,299 | CTCCAGGTAAGGTGGAGAGTCCAGCA |
| TUR | 124,911,244 | 124,911,268 | CACAGCTAAAGAGACAGCCATAGAG |
| TDF | 124,904,903 | 124,904,924 | CATCTCGTTCTCGTTCTCATCC |
| TDP | 124,904,879 | 124,904,902 | TTGGTGCCCTCTCCCTGTTCCTTT |
| TDR | 124,904,845 | 124,904,865 | CTCCACCAGTGAAAGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGAAGGACTGGGTCTGGAG |
| SD | GCCAGTGCTTTCCTTCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12253A-A11 | VGB6 | C57BL/6NTac | |
| 12253A-B6 | VGB6 | C57BL/6NTac | |
| 12253A-B9 | VGB6 | C57BL/6NTac | |
| 12253A-F10 | VGB6 | C57BL/6NTac | |
| 12253A-G11 | VGB6 | C57BL/6NTac | |
| 12253A-D4 | VGB6 | C57BL/6NTac | |
