| Gene | Hmg20b |
| Chromosome | 10 |
| Deletion Start | 81,350,168 |
| Deletion End | 81,346,430 |
| Deletion Size | 3,739 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,349,497 | 81,349,517 | GGATGACGCTGGTTTCCATTG |
| TUP | 81,349,472 | 81,349,493 | CCAAGACCTGCGGGAGGCAAGA |
| TUR | 81,349,429 | 81,349,448 | TCCTGCTTGACAGCCACTAC |
| TDF | 81,347,123 | 81,347,145 | GGGTTGAGGCCAGAGATGAAATG |
| TDP | 81,347,093 | 81,347,120 | TCTATAGCACTCCGGGACCAGTATGACG |
| TDR | 81,347,058 | 81,347,076 | TGCGGGACAGGAGTCAGAG |
PCR Primers
| Primer | Sequence |
| SU | AATTAGAGCGCTATCCTTGG |
| SD | CCAGATTGAGTGATGAAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12255A-G5 | VGB6 | C57BL/6NTac | |
| 12255A-F9 | VGB6 | C57BL/6NTac | |
