| Gene | Gpr63 |
| Chromosome | 4 |
| Deletion Start | 25,007,281 |
| Deletion End | 25,008,560 |
| Deletion Size | 1,280 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,007,384 | 25,007,403 | CGGCTCCGCTACCATTTCAG |
| TUP | 25,007,405 | 25,007,428 | ATCCTAGCGCTGGTCCGCTGCTTA |
| TUR | 25,007,429 | 25,007,451 | AGTCATGGTTTCAAGGCTGTATC |
| TDF | 25,008,354 | 25,008,374 | CACCTGGCTACTCTGGCTCTG |
| TDP | 25,008,377 | 25,008,403 | ACCTCAAGTCTGCATTGAACCCACTGA |
| TDR | 25,008,426 | 25,008,443 | AAGCAGGCATCGTGGAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTGCAGCCACACTATCCTG |
| SD | CTGAGAGGCAGAGGGATTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12258A-C3 | VGB6 | C57BL/6NTac | |
| 12258B-D6 | VGB6 | C57BL/6NTac | |
| 12258C-A5 | VGB6 | C57BL/6NTac | |
| 12258C-C3 | VGB6 | C57BL/6NTac | |
| 12258C-D1 | VGB6 | C57BL/6NTac | |
| 12258C-B1 | VGB6 | C57BL/6NTac | |
