| Gene | Sertad1 |
| Chromosome | 7 |
| Deletion Start | 27,489,259 |
| Deletion End | 27,490,025 |
| Deletion Size | 767 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,489,548 | 27,489,565 | CTGTGGCAGACAGCCTTC |
| TUP | 27,489,566 | 27,489,589 | TGGCTAGCTCAGATGCTGGCCTCT |
| TUR | 27,489,621 | 27,489,643 | GGTTCAGGTCCTCAATGTGGTTG |
| TDF | 27,489,786 | 27,489,808 | CGATACCTCCATGTACGACAGTG |
| TDP | 27,489,809 | 27,489,835 | AACTTTGGTTACCAGCCTCTGAGGGTC |
| TDR | 27,489,850 | 27,489,868 | CCTTGGCTGGGCCATTCTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCAGAAGCTCACTGAGGAC |
| SD | TTGGTATTTCCCAAAGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12259A-B5 | VGB6 | C57BL/6NTac | |
| 12259A-E3 | VGB6 | C57BL/6NTac | |
