| Gene | 4930451C15Rik |
| Chromosome | 16 |
| Deletion Start | 17,561,208 |
| Deletion End | 17,549,343 |
| Deletion Size | 11,866 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,560,713 | 17,560,733 | ATGACCCGTGGACTGAGAATG |
| TUP | 17,560,687 | 17,560,711 | AGCTCAGTGTAGTGCTTGCCTAGCA |
| TUR | 17,560,639 | 17,560,661 | CCTCCAGCTTGCTGGTTAAGTTC |
| TDF | 17,549,842 | 17,549,863 | GTTGCAGGAACAACCGAATCTC |
| TDP | 17,549,815 | 17,549,839 | TGTCAGGAGCCCTGAAGTTGGGATT |
| TDR | 17,549,785 | 17,549,804 | ATCCGCAGCGTCTGATTGAC |
PCR Primers
| Primer | Sequence |
| SU | GAGCCGCTGTCTACTCCAAG |
| SD | GACTGGCCTCTTAACTTGGT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12263A-D1 | VGB6 | C57BL/6NTac | |
| 12263A-D5 | VGB6 | C57BL/6NTac | |
| 12263A-D10 | VGB6 | C57BL/6NTac | |
| 12263A-G3 | VGB6 | C57BL/6NTac | |
| 12263A-H9 | VGB6 | C57BL/6NTac | |
