| Gene | Rab13 |
| Chromosome | 3 |
| Deletion Start | 90,220,902 |
| Deletion End | 90,225,653 |
| Deletion Size | 4,752 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,221,305 | 90,221,327 | CGTCATTTCCATCTGCTTCCTTC |
| TUP | 90,221,333 | 90,221,357 | CCCATCACTGCACCTAGACGCTCTA |
| TUR | 90,221,399 | 90,221,419 | AGGTCAGTAAGAGGCCAACAC |
| TDF | 90,224,891 | 90,224,909 | GCCGGAGATCGGTGAGTTG |
| TDP | 90,224,916 | 90,224,937 | CTCGGGCTGAGTACCACTGCGC |
| TDR | 90,224,948 | 90,224,966 | TCGCCAGTTGCTTCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCCTTTGAATAGCGCCTC |
| SD | TGAACCAATATCTTGGCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12265A-F5 | VGB6 | C57BL/6NTac | |
| 12265A-A9 | VGB6 | C57BL/6NTac | |
| 12265A-H1 | VGB6 | C57BL/6NTac | |
| 12265A-B3 | VGB6 | C57BL/6NTac | |
