12267   Zfp161     Status: ES cell colonies screened / QC positives

GeneZfp161
Chromosome17
Deletion Start69,385,505
Deletion End69,388,695
Deletion Size3,191
Genome Build38
MGI IDMGI:1195345
NCBI Gene ID22666
BAC ID134E14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF69,386,32469,386,346GACTTTTAACTGGATGGCTGGAA
TUP69,386,34869,386,374TCTGGCATCCTATGAGCATAAAGCCAC
TUR69,386,37669,386,397GACCTCTGGGAAGACAGTGATG
TDF69,387,85869,387,877AGTCAAGAGGACGGCAAGTC
TDP69,387,87869,387,901TCCCACAACCACTCTCAGGGTTCA
TDR69,387,94569,387,963CCTGGCCGTAACAGTTCAC

PCR Primers

PrimerSequence
SUCAGCCGTCCGATATTCAAAC
SDCAGCTTGGCAAGAGTTGAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR74 bp++-
TDFTDR106 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12267C-A10VGB6C57BL/6NTac
12267C-C2VGB6C57BL/6NTac
12267C-E7VGB6C57BL/6NTac
12267C-D5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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