| Gene | Zbtb4 |
| Chromosome | 11 |
| Deletion Start | 69,775,874 |
| Deletion End | 69,778,353 |
| Deletion Size | 2,480 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,776,769 | 69,776,786 | TGAAGCGGCACAGCAATG |
| TUP | 69,776,789 | 69,776,811 | CACTCGTGGCGGAGGAAGTACCC |
| TUR | 69,776,818 | 69,776,840 | GGGCAAACACCTTCTCACAGTAG |
| TDF | 69,777,359 | 69,777,378 | GGGCAGTTACAGGTCTGAGG |
| TDP | 69,777,386 | 69,777,411 | ATGACAGGGCAAGATGAGGTACTGCC |
| TDR | 69,777,426 | 69,777,446 | GCAGGAACAGAGCCACTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CTACTTGCCCTTTGCTGTGG |
| SD | GTCTTGCACACCTTGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12268A-B2 | VGB6 | C57BL/6NTac | |
| 12268A-D5 | VGB6 | C57BL/6NTac | |
| 12268A-E10 | VGB6 | C57BL/6NTac | |
| 12268A-G5 | VGB6 | C57BL/6NTac | |
| 12268A-H8 | VGB6 | C57BL/6NTac | |
