| Gene | Pomgnt1 |
| Chromosome | 4 |
| Deletion Start | 116,151,127 |
| Deletion End | 116,159,337 |
| Deletion Size | 8,211 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,151,428 | 116,151,449 | GGCATCCAACAGAAACCAGAAG |
| TUP | 116,151,450 | 116,151,471 | TCTGGTCAGGCAGGCTTTGCCC |
| TUR | 116,151,507 | 116,151,527 | GGAGAGCCAGAGACACAGAAG |
| TDF | 116,158,510 | 116,158,531 | CCGAATGGAGACAGATGATGAC |
| TDP | 116,158,534 | 116,158,555 | CGCCACCTGGACTCAGCTTGCC |
| TDR | 116,158,581 | 116,158,601 | ATGTTGATGCGGGCGCTACTG |
PCR Primers
| Primer | Sequence |
| SU | GTTGAGCCAGATGGGAAGAG |
| SD | TTCCTGAGTATGAGCCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12271A-E1 | VGB6 | C57BL/6NTac | |
| 12271A-F10 | VGB6 | C57BL/6NTac | |
| 12271A-A1 | VGB6 | C57BL/6NTac | |
