| Gene | Sema4c |
| Chromosome | 1 |
| Deletion Start | 36,556,037 |
| Deletion End | 36,549,477 |
| Deletion Size | 6,561 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,555,359 | 36,555,381 | AGGAGACAGAGTGAACCAGACAG |
| TUP | 36,555,335 | 36,555,358 | CTCTCCTTTGCTTTCGCGCACACC |
| TUR | 36,555,286 | 36,555,307 | CCCAGGAGCACAGAGATGATAC |
| TDF | 36,549,718 | 36,549,738 | AGCCTCTGCCTTCTCCAACTC |
| TDP | 36,549,684 | 36,549,710 | CCTAGGAGGTGGTCGGAACTCAAATGC |
| TDR | 36,549,657 | 36,549,679 | CCCAACTGTAAACGCACATAACC |
PCR Primers
| Primer | Sequence |
| SU | CTTTAAGGCCAGAAGGAGCC |
| SD | GCTCCAAAGACTCAACCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12277A-B9 | VGB6 | C57BL/6NTac | |
| 12277A-D5 | VGB6 | C57BL/6NTac | |
| 12277A-D7 | VGB6 | C57BL/6NTac | |
| 12277A-F1 | VGB6 | C57BL/6NTac | |
| 12277A-G5 | VGB6 | C57BL/6NTac | |
| 12277A-H2 | VGB6 | C57BL/6NTac | |
