| Gene | Insm1 |
| Chromosome | 2 |
| Deletion Start | 146,222,269 |
| Deletion End | 146,223,873 |
| Deletion Size | 1,605 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 146,222,955 | 146,222,973 | CATCCGCAAGCTGCACTTC |
| TUP | 146,222,975 | 146,222,996 | AGGACGAGGTGACCACGTCGCC |
| TUR | 146,223,006 | 146,223,025 | GGCCCTCCTTGATCTTGAGC |
| TDF | 146,223,370 | 146,223,389 | GAGGACGGGCTCTACGAGTG |
| TDP | 146,223,391 | 146,223,414 | CACCACTGCGCCAAGAAGTTCCGT |
| TDR | 146,223,436 | 146,223,454 | GGTGATGTGCCAGCAGGTG |
PCR Primers
| Primer | Sequence |
| SU | CCATAGAGGAGCAGCAGACC |
| SD | ATTGGTAGGCAGAGCAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12281A-B3 | VGB6 | C57BL/6NTac | |
| 12281A-B6 | VGB6 | C57BL/6NTac | |
| 12281A-A5 | VGB6 | C57BL/6NTac | |
| 12281A-C2 | VGB6 | C57BL/6NTac | |
| 12281A-E4 | VGB6 | C57BL/6NTac | |
| 12281A-H1 | VGB6 | C57BL/6NTac | |
