| Gene | Gsx1 |
| Chromosome | 5 |
| Deletion Start | 147,188,893 |
| Deletion End | 147,190,164 |
| Deletion Size | 1,272 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,189,237 | 147,189,258 | TGCACTCTACCAGACCTCCTAC |
| TUP | 147,189,260 | 147,189,278 | CGCTGCCGGATCCCAGACA |
| TUR | 147,189,279 | 147,189,299 | CCCACAGAGATGCAGTGAAAC |
| TDF | 147,189,921 | 147,189,941 | CTGTCCGAGAAGCAGGTGAAG |
| TDP | 147,189,943 | 147,189,965 | TCTGGTTTCAGAACCGCCGGGTG |
| TDR | 147,189,983 | 147,190,002 | CGCGGTGGTTACTGCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGCGTAGCCCATCACATG |
| SD | CCAGATTGACCGAGAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12283A-B1 | VGB6 | C57BL/6NTac | |
| 12283B-H2 | VGB6 | C57BL/6NTac | |
| 12283B-B3 | VGB6 | C57BL/6NTac | |
