| Gene | Wdr31 |
| Chromosome | 4 |
| Deletion Start | 62,464,039 |
| Deletion End | 62,453,743 |
| Deletion Size | 10,297 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,463,135 | 62,463,155 | AACAGCCATGCCCTAAGACAG |
| TUP | 62,463,101 | 62,463,129 | AGGATTCCATGAGGACCTCACCCTTTGTC |
| TUR | 62,463,047 | 62,463,064 | GGACGAGGTGGATGGAAC |
| TDF | 62,453,881 | 62,453,900 | CCTGACCTCACTGGCTGTTG |
| TDP | 62,453,852 | 62,453,879 | TGACATCTCCTCCTTGTTATGCGCAAGT |
| TDR | 62,453,810 | 62,453,828 | TTGGCTGTGGTCCACTCTG |
PCR Primers
| Primer | Sequence |
| SU | GAGCATGCCTGTCTGTTCTTAG |
| SD | GAATCCAATCTCCAGAACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12285A-C5 | VGB6 | C57BL/6NTac | |
| 12285A-D1 | VGB6 | C57BL/6NTac | |
| 12285B-A7 | VGB6 | C57BL/6NTac | |
| 12285B-F9 | VGB6 | C57BL/6NTac | |
| 12285B-B5 | VGB6 | C57BL/6NTac | |
| 12285B-C4 | VGB6 | C57BL/6NTac | |
| 12285B-G1 | VGB6 | C57BL/6NTac | |
