| Gene | Tnfrsf18 |
| Chromosome | 4 |
| Deletion Start | 156,026,390 |
| Deletion End | 156,028,806 |
| Deletion Size | 2,417 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 156,026,456 | 156,026,474 | GTTGAGGAGCCTGGCTGTG |
| TUP | 156,026,482 | 156,026,507 | CAAGGTTCAGAACGGAAGTGGCAACA |
| TUR | 156,026,527 | 156,026,548 | CCTTGCCTTACCTGGAGCATAC |
| TDF | 156,028,639 | 156,028,658 | CAAGCCAGACGCTACAAGAC |
| TDP | 156,028,660 | 156,028,683 | TGCCCAGCTATACCCTTGGTGAGA |
| TDR | 156,028,695 | 156,028,713 | CCAGGGAAGGGTGCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GGCTCTCCTAGTTTCCCAGG |
| SD | TGTCCCTCAATGCAACTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12292A-E9 | VGB6 | C57BL/6NTac | |
| 12292A-G4 | VGB6 | C57BL/6NTac | |
| 12292A-H4 | VGB6 | C57BL/6NTac | |
