| Gene | Ccdc160 |
| Chromosome | X |
| Deletion Start | 52,798,086 |
| Deletion End | 52,799,076 |
| Deletion Size | 991 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,798,194 | 52,798,214 | GCAGACAACCACAAGAGGATG |
| TUP | 52,798,227 | 52,798,256 | AACTTCTCCGTTAGGAACACTCAAGAAGGA |
| TUR | 52,798,275 | 52,798,298 | TTCTGCTATCTGGGAAGAATAGTC |
| TDF | 52,798,917 | 52,798,938 | GCCATCAAGAATGAGCTGAGAG |
| TDP | 52,798,939 | 52,798,965 | TCGAGAAGACCCTAAGGGTGAAGAACA |
| TDR | 52,798,974 | 52,798,993 | TGTCTTCCAAGCACCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TTTCTGTGCATACAGGTGCC |
| SD | ACAAGGTCACACAGTGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12295A-D3 | VGB6 | C57BL/6NTac | |
| 12295B-G9 | VGB6 | C57BL/6NTac | |
| 12295C-B8 | VGB6 | C57BL/6NTac | |
