| Gene | Cbr3 |
| Chromosome | 16 |
| Deletion Start | 93,683,328 |
| Deletion End | 93,690,779 |
| Deletion Size | 7,452 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,684,029 | 93,684,049 | TCTGCTGAGGACAGGAAATGG |
| TUP | 93,684,051 | 93,684,071 | TCGCCCGGACACAATTAGCGC |
| TUR | 93,684,080 | 93,684,100 | GCGGGTAGGAAACCCACTATC |
| TDF | 93,690,241 | 93,690,258 | CCCACACTGCATGGAGAC |
| TDP | 93,690,261 | 93,690,284 | CGGTGCACACCTCCGTACCTTCTG |
| TDR | 93,690,296 | 93,690,315 | ACGCCAGGAAAGCCCATCAC |
PCR Primers
| Primer | Sequence |
| SU | TGCATTCTGCAGAGAACGAC |
| SD | CACATTAACTGGTTGAAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12296A-D9 | VGB6 | C57BL/6NTac | |
| 12296B-A11 | VGB6 | C57BL/6NTac | |
| 12296B-D1 | VGB6 | C57BL/6NTac | |
