| Gene | Rab43 |
| Chromosome | 6 |
| Deletion Start | 87,811,541 |
| Deletion End | 87,792,518 |
| Deletion Size | 19,024 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,811,100 | 87,811,120 | CGAAACAACTTGCGGTGGAAG |
| TUP | 87,811,076 | 87,811,097 | AACCTGCGGGCCGTTCCGTGAG |
| TUR | 87,811,009 | 87,811,030 | GTCGTTATTTGGCTGAGTGCTC |
| TDF | 87,792,724 | 87,792,743 | CGCAAAGGACTCCAGCAATG |
| TDP | 87,792,698 | 87,792,720 | AGGAGGCCTTCACCAGAGTGGCC |
| TDR | 87,792,636 | 87,792,656 | TGGATGTGGTCTGTGTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGGTGTTGGTTATGGGAGG |
| SD | GCAGGAGAATGACCTGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12297A-E5 | VGB6 | C57BL/6NTac | |
| 12297A-E7 | VGB6 | C57BL/6NTac | |
| 12297A-G5 | VGB6 | C57BL/6NTac | |
| 12297A-E12 | VGB6 | C57BL/6NTac | |
| 12297A-H12 | VGB6 | C57BL/6NTac | |
| 12297A-E10 | VGB6 | C57BL/6NTac | |
