| Gene | Cldn19 |
| Chromosome | 4 |
| Deletion Start | 119,255,576 |
| Deletion End | 119,259,863 |
| Deletion Size | 4,288 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,255,981 | 119,256,002 | GAGGAGGCAGGAACACAAGAAG |
| TUP | 119,256,007 | 119,256,030 | CAGCCTGGGCTCCCGGTCCTAATA |
| TUR | 119,256,035 | 119,256,055 | AGAACGCAAGGGTTGAATGAG |
| TDF | 119,259,479 | 119,259,496 | AGGCGTGGCTCTCCAATC |
| TDP | 119,259,497 | 119,259,521 | TGTGAGAGTAGGCCTCAGCCTCTGA |
| TDR | 119,259,552 | 119,259,574 | CCTCTGACCTCGTGACCTGTATG |
PCR Primers
| Primer | Sequence |
| SU | TATTGAGACAGGGAGGGCAG |
| SD | GTTGGGCCTCACAGAGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12298A-A12 | VGB6 | C57BL/6NTac | |
| 12298A-C9 | VGB6 | C57BL/6NTac | |
| 12298A-E6 | VGB6 | C57BL/6NTac | |
| 12298A-F6 | VGB6 | C57BL/6NTac | |
