| Gene | 4930548H24Rik |
| Chromosome | 5 |
| Deletion Start | 31,485,931 |
| Deletion End | 31,488,220 |
| Deletion Size | 2,290 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,486,685 | 31,486,705 | CAAAGAAGTCAGGGCCAGGAG |
| TUP | 31,486,708 | 31,486,732 | CAAGACAGGAACCTGGAGGCAGGAA |
| TUR | 31,486,760 | 31,486,782 | GAAAGCAGTCCAAGCAACTGAAC |
| TDF | 31,487,777 | 31,487,798 | AGAGAAGGAACTGGAGGATGTG |
| TDP | 31,487,800 | 31,487,825 | AAGCTGAGACGGCTCGGAAGGACCAC |
| TDR | 31,487,830 | 31,487,850 | GCTGCAAGAACTGCAAGTGTG |
PCR Primers
| Primer | Sequence |
| SU | CTTCTGGACTCTCGCCATTC |
| SD | ACACCACCCAGTTTCGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12302A-D12 | VGB6 | C57BL/6NTac | |
| 12302A-H11 | VGB6 | C57BL/6NTac | |
| 12302B-H10 | VGB6 | C57BL/6NTac | |
