| Gene | Pou3f2 |
| Chromosome | 4 |
| Deletion Start | 22,488,128 |
| Deletion End | 22,486,748 |
| Deletion Size | 1,381 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,487,554 | 22,487,574 | CAACGGCGGTTTGCTCTATTC |
| TUP | 22,487,529 | 22,487,551 | AGCCGAGCTTCACGGTGAACGGC |
| TUR | 22,487,450 | 22,487,468 | CTGCATGGTGTGGCTCATC |
| TDF | 22,487,217 | 22,487,235 | CCTGTACGGCAACGTGTTC |
| TDP | 22,487,195 | 22,487,216 | TCGCAGACCACCATCTGCAGGT |
| TDR | 22,487,121 | 22,487,142 | TGCCTCTTCCAACCACTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | GTAAATCAAAGGGCGCAGAG |
| SD | TGTCAACTTTGAGCATTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12303A-A4 | VGB6 | C57BL/6NTac | |
| 12303A-A9 | VGB6 | C57BL/6NTac | |
| 12303A-E1 | VGB6 | C57BL/6NTac | |
| 12303A-G5 | VGB6 | C57BL/6NTac | |
| 12303A-D4 | VGB6 | C57BL/6NTac | |
