| Gene | Tax1bp3 |
| Chromosome | 11 |
| Deletion Start | 73,177,307 |
| Deletion End | 73,181,302 |
| Deletion Size | 3,996 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,178,219 | 73,178,241 | CCTTCTTAGCCTTCCAAGTGTTG |
| TUP | 73,178,245 | 73,178,271 | TCACAGGTGTGACTTGTGTCCACAGTC |
| TUR | 73,178,275 | 73,178,295 | CAAGGCCCATCCCTACAGTTC |
| TDF | 73,180,738 | 73,180,758 | ACGTCACACGAGTATCAGAGG |
| TDP | 73,180,766 | 73,180,788 | TGCTGAAATTGCTGGGCTGCAGA |
| TDR | 73,180,794 | 73,180,814 | CCGTACCTGCATGATCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TCGCGGAGTTACCTGAGAC |
| SD | GCATAGGGAGAGGCACTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12316A-H2 | VGB6 | C57BL/6NTac | |
| 12316A-H4 | VGB6 | C57BL/6NTac | |
| 12316A-C10 | VGB6 | C57BL/6NTac | |
